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The Thai Journal of Veterinary Medicine

Abstract

We evaluated propofol overdose for euthanasia of zebrafish (Danio rerio). We hypothesized that 1) propofol 40 mg/L at 10 min submersion euthanizes zebrafish as effective as 20 or 30 min submersion; 2) propofol 80 mg/L at 10 min submersion euthanizes zebrafish as effective as with 100 or 120 mg/L. Wild-type AB zebrafish were randomly submersed into: Exp.1 - propofol 40 mg/L for 10, 20, or 30 mins; or Exp.2 - 80, 100, or 120 mg/L for 10 mins. Criteria monitored: aversive behavior; time to loss of righting reflex (LORR); undulation cessation; operculation cessation; fish movement from tank tap reflex cessation; and euthanasia rate. Results: No aversive behavior noted; LORR 5 sec for both experiments; undulation cessation: 22 sec (Exp.1), 5 sec (Exp.2); operculation cessation: 66 sec (Exp.1), 51 sec (Exp.2); fish movement from tank tap reflex cessation: 13 min in the 20 and 30 min groups, and fish still moved in the 10 min group (Exp.1), no fish movement in any groups (Exp.2); euthanasia rate: 30% in 10 min group and 100% in 20 and 30 min group (Exp.1); 90% in 80 mg/L group, 50% in 100 mg/L group and 100% in 120 mg/L group (Exp.2). Propofol 40 mg/L at 10 min submersion does not euthanize zebrafish as effective as 20 or 30 min submersion; 2) propofol 80 or 100 mg/L at 10 min submersion does not euthanize zebrafish as effective as 120 mg/L. Propofol at 40 mg/L for at least 20 min or 120 mg/L for 10 min submersion effectively euthanizes zebrafish.

DOI

10.56808/2985-1130.3070

First Page

13

Last Page

16

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