The Thai Journal of Veterinary Medicine


Staphylococcus aureusis the leading cause of mastitis in dairy goats and is globally recognized as a significant mastitis-causing pathogen in dairy animals. Previous studies have established it as the causative agent of various human disorders, including food poisoning and Staphylococcal enterotoxins. Furthermore, poor sanitary conditions suit infection by this bacterium. This study determined the occurrence of S. aureusin infected dairy goats by molecular analysis. A hundred raw milk samples were collected from two infected dairy goat breeds (Beetal = 71; Teddy = 29) and cultured on blood agar media. The strain identified as S. aureusby morphological method (Gram staining), biochemical tests (catalase and coagulase) and further identified through molecular method using the 16SrRNAgene. Overall, 58 (Beetal = 45; Teddy = 13) out of 100 raw milk samples (58%) were found to be positive for S. aureus. Further, ten samples of fresh milk positive for other microbial species includingS. hominis, S. capitis and S. lentus were isolated. The 16SrRNAgene was sequenced of fifteen S. aureusisolates representing various geographical regions. Phylogenetic analysis was performed based on the 16SrRNAgene. In conclusion, S. aureus was more prevalent in the raw milk samples of the infected goats and acted as an etiologic agent of mastitis in the dairy goats.Hence, more measures that are hygienic should be implemented to improve milk quality and to prevent S. aureuscontamination.

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