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The Thai Journal of Veterinary Medicine

Abstract

In 2014, porcine epidemic diarrhea (PED) outbreaks characterized by mild clinical disease and low preweaning mortality increased in frequency in Thailand. An outbreak investigation, based on the detection of the classical variant and the allowance of the herd owners, was conducted in 5 swine herds and 25 intestinal samples were submitted from herds with outbreaks of diarrhea for sequencing analysis. PEDV isolation and complete nucleotide sequences of the spike (S) glycoprotein were determined. The PEDV isolate EAS1 was identified. Phylogenetic analysis based on the full-length spike gene demonstrated that EAS1 belonged to the classical variant group with high nucleotide and amino acid identity (98.4-99.0% and 96.6-97.9%) with LZC and SM98, which are the Chinese and Korean classical variant PEDVs, respectively. The results suggest the emergence of a classical variant PEDV responsible for the mild clinical disease in Thailand. The introduction could have been due to the use of illegally imported modified live vaccines from other countries.

DOI

10.56808/2985-1130.2959

First Page

663

Last Page

669

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