The Thai Journal of Veterinary Medicine


Oxidative stress can result from either the excessive production of reactive oxygen species (ROS) or an impaired antioxidant system, or both. It causes damage to lipids, proteins and DNA. Therefore, oxidative stress may be involved in carcinogenesis, and is associated with many types of cancer in dogs. The objective of this study was to compare the levels of malondialdehyde, protein hydroperoxides, glutathione, retinol and alpha-tocopherol between dogs with mast cell tumors and clinically healthy controls. Blood samples were obtained from eighteen clinically healthy dogs and fourteen dogs with spontaneous mast cell tumors. Malondialdehyde and protein hydroperoxides levels were measured by the thiobarbituric acid reactive substance assay, and the ferric-xylenol orange assay, respectively. Glutathione level was determined using spectrophotometric assay. Retinol and alpha-tocopherol levels were measured using the high performance liquid chromatographic method. Dogs with mast cell tumors had significantly higher levels of malondialdehyde (P<0.01) and protein hydroperoxides (P<0.05) compared with the clinically healthy controls. When considering antioxidants, dogs with mast cell tumors had significantly lower levels of glutathione (P<0.01), retinol (P<0.05) and alpha-tocopherol (P<0.01) compared with the clinically healthy controls. Mast cell tumors in dogs are associated with oxidative stress and antioxidant status. Further studies on oxidative stress and antioxidant activity in dogs should be conducted to guide and plan the complementary treatment of canine cancer.

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