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The Thai Journal of Veterinary Medicine

Abstract

The potential inhibitory effect of Magnolia officinalis extract (MOE) on Helicobacter pylori was studied by using (i) disk agar diffusion assay, (ii) in vitro inhibition assays of H. pylori adhesion to human gastric adenocarcinoma epithelial cell line AGS cells, (iii) inhibition of H. pylori-induced inflammation in AGS cell, and (iv) in vivo H. pylori SS1 mouse model of infection over a period of 4 weeks. Results indicated that MOE had a wide range of inhibitory effect against H. pylori growth. The inhibition of H. pylori adherence to gastric epithelial cells was identified by MOE. In addition, MOE inhibited H. pylori-induced inflammatory response significantly by reduced interleukin (IL)-8 expressions in H. pylori-infected AGS cells. Also, significant inhibition activity of MOE against H. pylori was identified in a mouse model. In vivo experiments were performed by per oral inoculation of MOE over a period of 4 weeks to fifty C57BL/6 mice previously infected with H. pylori SS1. Rapid urease tests of the mice stomachs demonstrated a significant reduction in H. pylori colonization. In addition to the therapeutic effect against H. pylori infection, the MOE reduced mucosal inflammation and epithelial damages in the stomach of the H. pylori-infected mice. These results demonstrate that the MOE successfully cured H. pylori infection and prevented H. pylori-induced pathology. Therefore, MOE could be a promising treatment for patients with gastric complaints including gastritis caused by H. pylori.

Publisher

Faculty of Veterinary Science, Chulalongkorn University

First Page

203

Last Page

210

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