The Thai Journal of Veterinary Medicine


Rabies virus (RV) phosphoprotein (P) is a multifunctional protein. However, the functions of this protein are not fully understood. To further investigate this question, the P protein was expressed and used to raise monospecific polyclonal antibodies. The gene coding for P protein was cloned into expression vector pET-32a (+) to yield pET-32a (+)-P. His-tagged P protein was expressed in E. coli BL21 cells and analyzed by SDS-PAGE. After purification by nickel affinity chromatography under native conditions, the recombinant P protein was used to raise anti-P polyclonal antibodies in mice. Western blot analysis showed that the P protein was recognized by the polyclonal antibodies. Immunofluorescence assays also showed that the antibody was able to recognize the native P protein in RV-infected cells.

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