The effect of low-dose chemotherapy on tumor motility of canine oral malignant melanoma has been investigated on an in vitro model using low-dose cyclophosphamide and piroxicam. Cyclophosphamide, at a concentration of 0.1 mg/mL in culture medium, significantly inhibited tumor cell migration/invasion through trans-well assays and suppressed both ERK and NF-κB signaling, the latter of which was observed through IκB-α and P-IκBα expression. Furthermore, in combination with a COX-2 inhibitor, i.e. piroxicam (0.38 mg/mL in culture medium), cyclophosphamide revealed a potential anti-migration effect over the single piroxicam treatment on a wound assay. This result suggested that low-dose cyclophosphamide might have influence on tumor cell motility by inhibition of the NF-κB/ERK cascade.
Faculty of Veterinary Science, Chulalongkorn University
Choisunirachon, Nan; Yoshida, Kota; Saeki, Kohei; Tanaka, Yuiko; Mochizuki, Manabu; Nishimura, Ryohei; Sasaki, Nobuo; and Nakagawa, Takayuki
"Low-Dose Cyclophosphamide and Piroxicam Inhibit Growth, Migration, and Invasion of Canine Oral Malignant Melanoma Cell Line,"
The Thai Journal of Veterinary Medicine: Vol. 45:
3, Article 8.
Available at: https://digital.car.chula.ac.th/tjvm/vol45/iss3/8