Low-Dose Cyclophosphamide and Piroxicam Inhibit Growth, Migration, and Invasion of Canine Oral Malignant Melanoma Cell Line

Authors

Nan Choisunirachon
Kota Yoshida
Kohei Saeki
Yuiko Tanaka
Manabu Mochizuki
Ryohei Nishimura
Nobuo Sasaki
Takayuki Nakagawa

Abstract

The effect of low-dose chemotherapy on tumor motility of canine oral malignant melanoma has been investigated on an in vitro model using low-dose cyclophosphamide and piroxicam. Cyclophosphamide, at a concentration of 0.1 mg/mL in culture medium, significantly inhibited tumor cell migration/invasion through trans-well assays and suppressed both ERK and NF-κB signaling, the latter of which was observed through IκB-α and P-IκBα expression. Furthermore, in combination with a COX-2 inhibitor, i.e. piroxicam (0.38 mg/mL in culture medium), cyclophosphamide revealed a potential anti-migration effect over the single piroxicam treatment on a wound assay. This result suggested that low-dose cyclophosphamide might have influence on tumor cell motility by inhibition of the NF-κB/ERK cascade.

DOI

10.56808/2985-1130.2661

First Page

381

Last Page

387

Recommended Citation

Choisunirachon, Nan; Yoshida, Kota; Saeki, Kohei; Tanaka, Yuiko; Mochizuki, Manabu; Nishimura, Ryohei; Sasaki, Nobuo; and Nakagawa, Takayuki (2015) "Low-Dose Cyclophosphamide and Piroxicam Inhibit Growth, Migration, and Invasion of Canine Oral Malignant Melanoma Cell Line," The Thai Journal of Veterinary Medicine: Vol. 45: Iss. 3, Article 8.
DOI: https://doi.org/10.56808/2985-1130.2661
Available at: https://digital.car.chula.ac.th/tjvm/vol45/iss3/8