MicroRNAs (miRNAs) are small non coding RNA that regulate gene expression as post transcriptional regulators in mammalian tissues. One of the miRNAs that participates in mammalian immune response is miR-29a. Despite the limited information of miR-29a in porcine cells, it is interesting to determine whether miR-29a involves in immune regulation in pig. In this study, we developed a quantitative reverse transcription polymerase chain reaction using SYBR detection system to examine the expression of primary miR-29a (pri-miR-29a) in porcine peripheral blood mononuclear cells (PBMCs) in the presence of mitogen. PBMCs were stimulated with concanavalin A (Con A) at a concentration of 10 μg/ml for 12 and 24 hours. Results showed that the expression level of pri-miR-29a decreased significantly in Con A stimulated PBMCs, while interferon-gamma (IFN-γ) increased relatively to the untreated control cells at 12 and 24 hour. In conclusion, our findings demonstrate the inversed expression patterns of pri-miR-29a and IFN-γ in Con A stimulated porcine PBMCs. Future studies of the function of miR-29a should provide important insight into the roles of this microRNA in pig immune response.
Nantakhruea, Sunaree; Lekcharoensuk, Porntippa; and Surachetpong, Win
"Analysis of miRNA-29a Expression in Porcine Peripheral Blood Mononuclear Cells Using Quantitative Reverse Transcription Polymerase Chain Reaction,"
The Thai Journal of Veterinary Medicine: Vol. 43:
4, Article 16.
Available at: https://digital.car.chula.ac.th/tjvm/vol43/iss4/16