The Thai Journal of Veterinary Medicine


Pulsed-field gel electrophoresis (PFGE) and Random amplified polymorphic DNA (RAPD) analyses were performed to compare techniques and to investigate genetic diversity as an epidemiological data of Thai M. hyosynoviae isolates. A total of 42 isolates including a type strain S16 was typable and consisted of 39 different patterns by RAPD technique, whereas 37 isolates (97%) were typable and consisted of 22 different patterns by PFGE technique. Based on PFGE patterns, multiple clones of M. hyosynoviae were generally present in pig farms, whereas high genetic heterogeneity of M. hyosynoviae among the pig farms was shown. No identical PFGE pattern between the pig farms was found except two farms that were located in the same province. This finding might indicate the distribution of the organism from the same source. Monitoring the genetic diversity of M. hyosynoviae strains using PFGE analysis should be useful to elucidate the epidemiology of M. hyosynoviae infections in Thailand.



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