Buccal Swab as a Source of Noninvasive Technique for Genomic DNA Collection in Felidae

Authors

Janjira Phavaphutanon
Sudtisa Laopium
Kawin Nanklang
Kaikanoke Sirinarumitr
Kornchai Kornkaewrat
Anuchai Pinyopummin
Jitrakorn Viriyarumpa
Piyawan Suthanmapinunt
Narathip Vorawattanatham

Abstract

This study was conducted to determine whether buccal cells obtained by swabbing could be a DNA source for genetic analysis, and to compare two preservative media, distilled water and Tris-EDTA (TE), pH 8.0. Buccal cell samples were collected from 30 domestic cats (Felis catus), 4 captive fishing cats (Prionailurus viverrinus) and 8 captive tigers (Panthera tigris). The total concentration of DNA and purity were not statistically different between the preservative media. All samples could be graded as a minimum as high quality DNA (DNA found with A260/A280 ratio >2.0 or < 1.8). In addition, the quality of DNA extracted from both media was successfully amplified for PCR. However, when compared to whole blood samples, total DNA concentration from buccal swab was significantly lower (p < 0.05) .Despite the decrease in DNA yield, buccal swab could be an alternative source for obtaining DNA for genetic analyses. This non-invasive, simple and inexpensive technique eases genetic studies in this family especially in domestic cat from which whole blood samples are difficult to collect.

DOI

10.56808/2985-1130.2506

First Page

455

Last Page

460

Recommended Citation

Phavaphutanon, Janjira; Laopium, Sudtisa; Nanklang, Kawin; Sirinarumitr, Kaikanoke; Kornkaewrat, Kornchai; Pinyopummin, Anuchai; Viriyarumpa, Jitrakorn; Suthanmapinunt, Piyawan; and Vorawattanatham, Narathip (2013) "Buccal Swab as a Source of Noninvasive Technique for Genomic DNA Collection in Felidae," The Thai Journal of Veterinary Medicine: Vol. 43: Iss. 3, Article 19.
DOI: https://doi.org/10.56808/2985-1130.2506
Available at: https://digital.car.chula.ac.th/tjvm/vol43/iss3/19