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The Thai Journal of Veterinary Medicine

Abstract

Laparoscopic artificial insemination (LAI) and embryo transfer (ET) were used to produce cross-bred blackcolored goat. In Experiment 1, LAI with frozen-thawed semen of black buck (Australian Melaan) was performed in 75% Saanen cross-bred does (white color, n = 70). The total numbers of 68 kids were born from 50 does. The skin colors of kids born were black (10.29%), white (39.71%) and other colors (50%). In Experiment 2, two cross-breeding programs were tested including program I: frozen semen of Australian Melaan inseminated to Black Bengal female (n = 7) and program II: frozen semen of Black Bengal inseminated to 50% Australian Melaan (n = 7). For embryo transfer program, the donors were superovulated and inseminated through laparoscopy with frozen semen at 21 hours after estrus. Thirty embryos at 4-8 cell stages (day 3) were surgically collected and transferred into 30 recipients (75% Saanen cross-breed) at approximately 60 hours following LAI. Pregnancy rates were 30%. Nine kids born from both programs were black in color with 2.56±0.95 kg birth weight. It is concluded that laparoscopic insemination and embryo transfer can be successfully combined to produce and sustain the genetic potential encoding the black colored skin.

Publisher

Faculty of Veterinary Science, Chulalongkorn University

First Page

259

Last Page

263

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