To study the subchronic and chronic lung effects of low dose of Diesel Exhaust Particles (DEPs), mice were intratracheally instilled with 50 μg of DEPs. Cellular and biochemical parameters in bronchoalveolar lavage fluid (BALF) and histological alteration were determined at 90, 120, 150 and 180 days after instillation. From BALF results, exposure to 50 μg of DEPs did not cause any pulmonary inflammation. However, mild to moderate pulmonary changes evidenced by multifocal DEPs laden alveolar macrophages (AMs) aggregation, type II alveolar cell proliferation, thickening of alveolar wall, interstitial fibrosis and edema were observed in treated mouse lungs. Basement (BM) damage detected by laminin immunohistochemistry and myofibroblast proliferation detected by smooth muscle actin immunoreactivity on the lungs remained in the alveolar region until chronic stage. Metallothionein expression in DEPs-treated mice occurred in lung epithelial cells and laden AMs, which correlated with lung lesions. These findings suggest that instillation of a low dose of DEPs causes mild to moderate pulmonary changes and tissue damage related to the existence of DEPs.
Kaewamatawong, Theerayuth; Banlunara, Wijit; Bintvihok, Anong; and Shimada, Akinori
"Subchronic to Chronic Pulmonary Effects of Low Dose of Diesel Exhaust Particles in Mice after Single Intratracheal Instillation: Pathological Changes and Metallothionien Responses,"
The Thai Journal of Veterinary Medicine: Vol. 43:
1, Article 14.
Available at: https://digital.car.chula.ac.th/tjvm/vol43/iss1/14