The Thai Journal of Veterinary Medicine
Article Title
Abstract
The present study aimed to develop a quantitative method for the detection of chicken infectious anemia virus (CIAV). A real time polymerase chain reaction (real-time PCR) was developed to determine CIAV. The developed real-time PCR was a rapid and reliable quantitative technique for CIAV DNA compared with the conventional PCR. The appropriate cut-off was at the 40th cycle. Results indicated that the CT value of 39.62 was shown at the 10-9 dilution or 0.454 fg/μl or 3.86 x 103 DNA copies. This data suggests that the limit of detection for real-time PCR is 0.454fg/μl. The quantitative real-time PCR will be useful for studying CIAV pathogenesis and determining the viral yield.
Publisher
Faculty of Veterinary Science, Chulalongkorn University
First Page
533
Last Page
536
Recommended Citation
Chansiripornchai, Niwat; Wanasawaeng, Wisanu; Wongchidwan, Nantawan; Chaichote, Supawadee; and Sasipreeyajan, Jiroj
(2012)
"Application of Real-time Polymerase Chain Reaction for Quantitative Detection of Chicken Infectious Anemia Virus,"
The Thai Journal of Veterinary Medicine: Vol. 42:
Iss.
4, Article 18.
Available at:
https://digital.car.chula.ac.th/tjvm/vol42/iss4/18