Application of Real-time Polymerase Chain Reaction for Quantitative Detection of Chicken Infectious Anemia Virus

Authors

Niwat Chansiripornchai
Wisanu Wanasawaeng
Nantawan Wongchidwan
Supawadee Chaichote
Jiroj Sasipreeyajan

Abstract

The present study aimed to develop a quantitative method for the detection of chicken infectious anemia virus (CIAV). A real time polymerase chain reaction (real-time PCR) was developed to determine CIAV. The developed real-time PCR was a rapid and reliable quantitative technique for CIAV DNA compared with the conventional PCR. The appropriate cut-off was at the 40th cycle. Results indicated that the CT value of 39.62 was shown at the 10-9 dilution or 0.454 fg/μl or 3.86 x 103 DNA copies. This data suggests that the limit of detection for real-time PCR is 0.454fg/μl. The quantitative real-time PCR will be useful for studying CIAV pathogenesis and determining the viral yield.

DOI

10.56808/2985-1130.2435

First Page

533

Last Page

536

Recommended Citation

Chansiripornchai, Niwat; Wanasawaeng, Wisanu; Wongchidwan, Nantawan; Chaichote, Supawadee; and Sasipreeyajan, Jiroj (2012) "Application of Real-time Polymerase Chain Reaction for Quantitative Detection of Chicken Infectious Anemia Virus," The Thai Journal of Veterinary Medicine: Vol. 42: Iss. 4, Article 18.
DOI: https://doi.org/10.56808/2985-1130.2435
Available at: https://digital.car.chula.ac.th/tjvm/vol42/iss4/18