The Thai Journal of Veterinary Medicine
Effects of Semen Extender on Motility and Movement Patterns of Frozen-thawed Eld’s Deer (Cervus eldii) Spermatozoa
Two semen samples of Siamese Eld,s deer (Cervus eldii siamensis: SED) and seven semen samples of Thamin Eld,s deer (Cervus eldii thamin: TED) were collected by using electroejaculation. Each ejaculate was diluted and cryopreserved with 2 extenders; 1) TRIS; and 2) modified BF5F supplemented with 5% final glycerol concentration. The movement patterns of frozen-thawed spermatozoa were assessed using computer-assisted sperm analysis (CASA). In TED, the results revealed a significantly higher (p< 0.05) percentage of total motility (%TM), percentage of progressive motility (%PM), track speed (VCL) and lateral amplitude (ALH) in TRIS extender. The results also revealed a significantly low (p< 0.05) progressive velocity (VSL), straightness (STR) and linearity (LIN) in TRIS extender. However, for path velocity (VAP) and beat frequency (BCF), there were no differences between both extenders. In SED, sperm motility and sperm movement parameters did not show differences between TRIS and modified BF5F extenders. Findings from this study will aid in Eld’s deer semen banking and artificial insemination success in the future.
Faculty of Veterinary Science, Chulalongkorn University
Rittem, Sukumal; Thongthainun, Daraka; Tipkantha, Wanlaya; Siriaroonrat, Boripat; and Thongtip, Nikorn
"Effects of Semen Extender on Motility and Movement Patterns of Frozen-thawed Eld’s Deer (Cervus eldii) Spermatozoa,"
The Thai Journal of Veterinary Medicine: Vol. 42:
4, Article 17.
Available at: https://digital.car.chula.ac.th/tjvm/vol42/iss4/17