The Thai Journal of Veterinary Medicine


Contamination of the environment with Nanosilver particles (AgNPs) has caused considerable concern. Atpresent there is little information of AgNPs toxicity, especially in aquatic environments. The purpose of this studywas to evaluate toxic effects of AgNPs on Zebrafish (Danio rerio) embryonic development. Toxicity test of AgNPs onthe embryos was conducted in concentration of 0.05, 0.1, 0.5, 1 and 5 μg/ml. Standard water was used as control.Observation for mortality, morphological and functional abnormalities at 4, 24, 48 and 72 hours post fertilization (hpf)were performed. The results showed that AgNPs at 1 and 5 μg/ml had significant lethal effect on the embryos.Calculated LC50 at 48 hpf of AgNPs to the embryos was 1.78 μg/ml. In concentration of 1 μg/ml, decreasedmovement rate and hatchability of the embryos were significantly different when compared to the controls. Noremarkable toxic effect was found in the embryos that were exposed to 0.05 0.1 and 0.5 μg/ml AgNPs whencompared to the controls. In summary, colloidal AgNPs could be a potential cause of the adverse effects involvingmortality and altered physiological functions to aquatic embryos. Silver ion might play an important role in toxicmechanism.



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