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The Thai Journal of Veterinary Medicine

Abstract

This study was conducted to investigate Chikungunya virus (CHIKV) infection in mammal and avian byusing mice and baby chickens as model animals. Thailand 2010 and Ross/186 strain (reference strain) were used inthis study. 104, 106 and 108 CID50 of CHIKV were inoculated to four- and six-week-old mice. Blood was collected andtested for virus for seven days. 108 CID50 of CHIKV was inoculated to two-week-old mice. Blood was collected andtested for virus for five days. For the baby chickens, 102, 104, 106 and 108 CID50 of CHIKV were inoculated to five-dayoldbaby chickens. Blood was collected and tested for virus for seven days. Serum samples were tested for CHIKV byreverse transcription polymerase chain reaction. CHIKV was detected in two- and four-week-old mice that wereinoculated with 108 CID50 of CHIKV. The percentages of Thailand 2010 strain of CHIKV infection in four-week-oldmice were 80, 80, and 60% on days 1, 2, and 3 post inoculation (PI), respectively. The percentages of Ross/186 strainof CHIKV infection in four-week-old mice were 60, 100, and 60% on days 1, 2, and 3 PI, respectively. The percentagesof Thailand 2010 strain of CHIKV infection in two-week-old mice were 90, 100, and 67% on days 1, 2, and 3 PI,respectively. The percentages of Ross/186 strain of CHIKV infection in two-week-old mice were 100, 100, 50, 83, and100% on days 1, 2, 3, 4, and 5 PI, respectively. No virus was detected in any 6-week-old mice and baby chickens.

DOI

10.56808/2985-1130.2407

First Page

353

Last Page

358

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