Detection of Enzootic Bovine Leukosis in Cattle using Nested Polymerase Chain Reaction Assay

Authors

Jomelson Alfaro Uera
Jonathan Ventura Lazaro
Claro Niegos Mingala

Abstract

Enzootic bovine leukosis (EBL) is caused by bovine leukemia virus (BLV) infection. BLV was detected incattle using nested polymerase chain reaction (PCR) assay and were identified BLV infected cattle farms in fiveselected provinces in the Philippines. A total of 300 cattle blood samples were used. BLV Proviral DNA wasextracted and amplified using nested PCR assay targeting the BLV long terminal repeat (LTR). Results showed that11 samples (3.67%) of the 300 cattle blood samples used were positive for BLV infection. This study is considered firstreport of cattle EBL in the Philippines.

DOI

10.56808/2985-1130.2403

First Page

319

Last Page

324

Recommended Citation

Uera, Jomelson Alfaro; Lazaro, Jonathan Ventura; and Mingala, Claro Niegos (2012) "Detection of Enzootic Bovine Leukosis in Cattle using Nested Polymerase Chain Reaction Assay," The Thai Journal of Veterinary Medicine: Vol. 42: Iss. 3, Article 10.
DOI: https://doi.org/10.56808/2985-1130.2403
Available at: https://digital.car.chula.ac.th/tjvm/vol42/iss3/10