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The Thai Journal of Veterinary Medicine

Abstract

This present study was carried out to use conventional and real-time PCR for detection and segregation of Brucella abortus and Brucella melitensis in aborted bovine, ovine, caprine, buffalo and camel fetuses. All samples were collected and immediately transferred to laboratory, genomic DNA was extracted and the conventional and real-time PCR by specific primers for Brucella abortus and Brucella melitensis was performed. A TaqMan analysis and single-step PCR was carried out in total 3710 DNA of abomasal contents of aborted fetuses. In total, 281/892 (31.5%) bovine, 224/810 (27.65%) ovine, 219/786 (27.86%) caprine, 199/604 (32.94%) buffalo and 201/618 (32.52%) camel fetus samples gave positive results for Brucella species by conventional PCR. Moreover, 45/281 and 231/281, 169/224 and 49/224, 194/219 and 22/219, 57/199 and 137/199 and finally 51/201 and 143/201 specimens were positive for B. melitensis and B. abortus in aborted bovine, ovine, caprine, buffalo and camel fetuses by real-time PCR, respectively. The sensitivity and specificity of real-time PCR obtained 100% and 100%. Statistical analysis showed significant differences (p<0.01) between B. abortus and B. melitensis that were detected in abomasal contents of aborted bovine, ovine, caprine, buffalo and camelid fetuses and between presences of Brucella spp. in bovine with caprine, buffalo and camel aborted fetuses (p<0.05). The CT values obtained from real-time PCR had significant differences between aborted bovine, ovine, caprine, buffalo and camel fetuses for presence of B. abortus and B. melitensis. Results showed that the real-time PCR is considerably faster than current standard methods for isolation and segregation of Brucella spp.

DOI

10.56808/2985-1130.2361

First Page

13

Last Page

20

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