The Thai Journal of Veterinary Medicine


The objective of this study is to establish a polymerase chain reaction (PCR) laboratory for detection of multidrug resistance1 (MDR1) mutation in Thailand. Blood samples were collected from 67 dogs, namely 16 Collies, 9 Shetland Sheepdogs, 5 Border Collies, 6 Shih tzus, 13 German Shepherds and 18 mixed breeds. Genomic DNA was extracted and PCR was performed to amplify 341 and 577 bp fragments to detect homozygous wild-type (+/+) and homozygous mutant (-/-), respectively. For the heterozygous mutant (+/-), both PCR fragments would be presented. An analysis of DNA sequences encompassing the 4-base pair deletion in the coding region of MDR1 gene was performed to confirm different MDR1 genotypes. Nine Collies (56.2%) were heterozygous for the MDR1 mutation (carrier) and 7 dogs (43.8%) were homozygous for the mutant allele (affected). None of the studied Collies were homozygous for the normal allele (normal). One Shetland Sheepdog (11.1%) was heterozygous mutant. All the other breeds were homozygous normal. This research can be used for further studies to establish the PCR-based diagnostic test in suspected dogs.



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