The Thai Journal of Veterinary Medicine


The toxicity of Kameng (Eclipta prostrate Linn.) and Kradhuawean (Spilanthes acmella (Linn.) Murr.) onzebrafish (Danio rerio) embryos was investigated. An aqueous crude extract of the leaves of each plant was tested withnewly spawned embryos in 96 well plates. The observed effect was based on the toxicity endpoint composed of lethal,sublethal and malformation effects appearing at 24, 48 and 144 hours post-fertilization (hpf). The results suggest thatthe lowest observed effect concentrations (LOEC) for a lethal and sublethal effect of Eclipta prostrate Linn. at 48 hpfwere 1% and 0.1% while the no observed effect concentrations (NOEC) for the lethal and sublethal effects of theplants at 48 hpf were 0.01% and 0.1%, respectively. LOEC for malformation and sublethal effects of Spilanthes acmellaLinn. Murr. at 48 hpf were 20% and 10% while NOEC for lethal, malformation and sublethal effects of S. acmella Linn.Murr. at 48 hpf were 20%, 10% and 1%, respectively. There was no lethal effect observable in the highestconcentration test to the embryo at 20% of Spilanthes acmella Linn. Murr.

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