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The Thai Journal of Veterinary Medicine

Abstract

As a cornified envelope protein, filaggrin (FLG) is involved in the formation and support of the skin barrier.FLG single nucleotide polymorphism (SNP) at the position 64,297,022 (rs22588227) on canine chromosome 17 hasinitially been described as being associated with canine atopic dermatitis (CAD) in Labrador Retrievers in UK. In thisstudy, we have examined whether the mentioned FLG SNP is associated with a susceptibility to CAD in small breeddogs, comprising 21 Poodles, 17 Shih tzus and 3 Pugs. Twelve of these subjects were dogs with atopy and wereassigned to the experiment group and the remaining 39 samples were healthy controls. The results showed nodifference of the allele frequencies at the above-mentioned position between dogs with atopic dermatitis and thecontrols. However, this study assessed a naturally observed sequence diversity of FLG in the dog, identifying 13 newSNPs within canine FLG and a novel repeated sequence of FLG which had not appeared in dog genome databases.Allele frequencies demonstrated that 2 of the 13 novel observed polymorphisms at the locations 64,297,000 (p = 0.041,odds ratio = 3.920) and 64,297,126 (p = 0.043, odds ratio = 3.706) were plausibly associated with a susceptibility toCAD. The effect of all SNPs was dependent on one another with a strong linkage disequilibrium (D’≥0.89) in onehaplotype block (frequencies ≥2%). This study suggests a role of FLG polymorphisms in CAD and also demonstratesthe successful attempts to identify another unique fragment of the repeated FLG sequence and the novel SNPs.However, since small population was included in this study, the study should be repeated with a larger population.

Publisher

Faculty of Veterinary Science, Chulalongkorn University

First Page

509

Last Page

518

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