Analysis on Differentially Expressed Genes of Muscle Tissues in Rugao Chicken at the Ages of 2 and 12 Weeks by Microarray

Authors

Qin De Luan
Guo Bin Chang
Ting Zhen
Zheng Yang Huang
Wei Zhou
Yan Liu
Guo Hong Chen

Abstract

Microarray containing 13319 probes was used to construct gene expression profiles in order to screendifferentially expressed genes of muscle tissues in Rugao chicken and investigate the molecular mechanism relatedwith muscle tissue traits at 2 and 12 weeks old of chicken. Two hundred and eight differentially expressed genes, ofwhich 108 were known genes, 94 up-regulated, 114 down-regulated were involved in growth, molecular mechanism,fat metabolism, cell proliferation, transcription and splicing factor, protein synthesis and degradation. Meanwhile,some genes of these differentially expressed genes that had no annotation in GenBank were screened out, they werepresumed to be unknown new genes. The differential expressions of 4 genes were confirmed by real-time quantitativePCR and the results indicated that the expression changes of these genes were generally consistent with the results ofGeneChip, a technology that could be used in breeding and selection of Chinese native chicken breeds.

DOI

10.56808/2985-1130.2313

First Page

299

Last Page

308

Recommended Citation

De Luan, Qin; Chang, Guo Bin; Zhen, Ting; Huang, Zheng Yang; Zhou, Wei; Liu, Yan; and Chen, Guo Hong (2011) "Analysis on Differentially Expressed Genes of Muscle Tissues in Rugao Chicken at the Ages of 2 and 12 Weeks by Microarray," The Thai Journal of Veterinary Medicine: Vol. 41: Iss. 3, Article 6.
DOI: https://doi.org/10.56808/2985-1130.2313
Available at: https://digital.car.chula.ac.th/tjvm/vol41/iss3/6