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The Thai Journal of Veterinary Medicine

Abstract

The aim of this study was to evaluate the effect of Equex STM Paste on goat semen quality aftercryopreservation. Semen samples were consecutively collected once a week, from six mature bucks. The semensamples with adequate motility (>60%) and sperm concentration (>1,500x106 sperm/ml) were frozen in an egg yolk-Tris semen extender supplemented either with (E+) or without (E-) Equex STM Paste. The semen quality includingmotility, viability, morphology, acrosome integrity and membrane functional integrity were evaluated beforeprocessing and after cryopreservation. Computer-assisted-semen analysis (CASA) was used to characterize the spermmotion patterns during the thermal resistance test at 0, 1, 2 and 3 hours post-thawing. After thawing, the spermmotility was higher in E+ group at 0 (p=0.0009), 1 (p>0.05) and 2 hours (p>0.05) compared to E- group. The positiveeffect of Equex was also observed in terms of viability (p=0.0002), normal tail (p<0.0001) and the percentage ofacrosome-intact spermatozoa (p>0.05). While semen in E- group exhibited better plasma membrane integrity, highernumber of normal sperm head, some of the motility characteristics seem better than that of E+ group but notsignificantly. In conclusion, the improvement of the quality of post thawed buck semen in extender supplementedwith Equex STM Paste was noticeable only in the first two hour after thawing

Publisher

Faculty of Veterinary Science, Chulalongkorn University

First Page

345

Last Page

352

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