The Thai Journal of Veterinary Medicine


An experiment was conducted to investigate the effect of Roselle calyx in two preparation forms in layerdiets on egg production performance, egg quality and TBARS value in plasma and yolk. Two hundred and seventy,33-week-old, CP Brown laying hens were randomly allocated into 6 treatments with 5 replications of 9 hens each.The dietary treatments were control diet, control diet supplemented with 250 mg/kg α-tocopheryl acetate, dietcontaining 1 and 2% of Roselle calyx crude extracts, and diet containing 2% and 4% Roselle calyx powder. Eggproduction performance was recorded, egg quality was measured and TBARS value in plasma and yolk wereanalyzed after fed experimental diets for 4 and 8 weeks. Yolk TBARS after storage for 10 and 20 days in refrigeratorand room temperature were also determined. The results demonstrated that there was no significant difference in eggproduction performance and egg quality and TBARS value in plasma and yolk at the 4th and 8th week amongtreatment groups (p>0.05). Roselle calyx tended to decrease TBARS value in yolk (p>0.05) while TBARS value in yolksignificantly increased when storage times increased (p<0.01) in both storage methods. In conclusion, both forms andlevels of Roselle had no adverse effect on egg production and egg quality. As an antioxidant, Roselle could not clearlyshow antioxidant activity. This may be due to the concentration of phenolic compound level in supplementedRoselle. Storage time was an important factor to decrease egg quality and increase TBARS value in yolk in bothstorage methods.

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