The Thai Journal of Veterinary Medicine


Paisan Tienthai


The glycosaminoglycans (GAGs) found in the mammalian oviductal tissue and luminal secretory fluidinfluence the fertilization processes in various patterns such as sperm storage, viability and capacitation. The aim ofthis study was to confirm the appearance of hyaluronan and syndecan-1 in the swamp buffalo oviduct consisting ofuterotubal junction (UTJ), isthmus, ampulla and infundibulum during oestrous cycle using histochemical andimmunohistochemical techniques. The non-sulphated GAGs, i.e. hyaluronan (HA), were noticeably localized in thesubepithelial connective tissue layer or lamina propria of all segments while the epithelial HA-positive staining wasdemonstrated only in the UTJ and isthmus (sperm reservoir) at the follicular phase. In contrast, the example of thesulphated GAGs, i.e. syndecan-1, were seen along the epithelial lining of all portions and both oestrous stages,however, the syndecan-1 positive labeled on the epithelial lining surface was intensely observed only in the UTJ andisthmus. Therefore, the present results established for the first time that non-sulphated and sulphated GAGs certainlycontained in the swamp buffalo oviductal tissue. The noticeable localization of HA in the UTJ and isthmus mightindicate the regulation of HA in the forming of sperm reservoir to maintain viability of spermatozoa before ovulationwhereas the expression of syndecan-1 may play a vital role in the regulation of sperm adhesion and the initiation ofsperm capacitation.



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