Cloning and Analysis of piRNAs from Testes of Three Species

Authors

Guobin Chang
Rong Chen
Ying Zhang
Aiqin Dai
Teng Ma
Guohong Chen

Abstract

piRNAs are a testis-specific class of small RNAs in mammal. Sixteen sequences of piRNAs were cloned fromthree different species by the method of construction of cDNA library including two sequences from mouse, eightsequences from quail and six sequences from chicken. Herein, the two piRNAs sequences from mouse had alreadybeen identified, while the others were cloned for the first time. The sequence analysis indicated that the avian piRNAshad a similar length and a preference of uridine for 5’-end with varying degrees to the mammalian piRNAs. Besidethe function in the process of sperm development and maturation, avian piRNAs may also have a role in regulatingthe development of nerve and tumor cells.

DOI

10.56808/2985-1130.2296

First Page

193

Last Page

198

Recommended Citation

Chang, Guobin; Chen, Rong; Zhang, Ying; Dai, Aiqin; Ma, Teng; and Chen, Guohong (2011) "Cloning and Analysis of piRNAs from Testes of Three Species," The Thai Journal of Veterinary Medicine: Vol. 41: Iss. 2, Article 9.
DOI: https://doi.org/10.56808/2985-1130.2296
Available at: https://digital.car.chula.ac.th/tjvm/vol41/iss2/9