The Thai Journal of Veterinary Medicine


The study aimed to apply flow cytometry (FC) for early detection and monitor of treated response cats withIMHA by measuring immunoglobulin-G (IgG) and immunoglobulin-M (IgM) on red blood cells. Coombs' test and FCmethod were performed in twenty-nine cats including: ten clinically normal client-owned cats and nineteen anemiccats with packed cell volume of less than 27%. Five anemic cats were positive for Coombs' test and had significantlyhigh levels of IgG and IgM on the red blood cells with high level of mean fluorescene intensity (MFI) measured by theFC method. In seven out of the fourteen anemic cats with negative Coombs’ results, the FC method demonstrated theincrease of MFI values for red blood cell-bound feline IgG and IgM. These seven cats were suspected of havingsubclinical IMHA. The FC method can provide the MFI value of the red blood cell-bound feline IgG and IgM whichcan be used for early detection and monitoring of the treated response in cats with IMHA.

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