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The Thai Journal of Veterinary Medicine

Abstract

Recently, we reported the preferential infection of a novel glial cell type from adult canine brain, termedSchwann cell-like brain glia (SCBG) with canine distemper virus (CDV). To further characterize virus-cell and cell-cellinteractions, SCBG in purified and mixed brain cell cultures was infected with different CDV strains. Cell cultureswere infected with the attenuated CDV-Ond, CDV-2544, CDV-OndeGFP, CDV-R252 and the mustelid virulent CDV-5804PeGFP strain. Cytopathic effects (CPEs) and the percentage of infection were monitored daily at 3 and 10 dayspost-infection (dpi), respectively. At 10dpi, the CDV strains induced mild to moderate and severe CPEs in purifiedSCBG and fibroblasts, respectively, while both cell types displayed mild to moderate CPE in mixed brain cell cultures.Comparative analysis of CDV infection revealed that the virulent CDV-5804PeGFP strain caused a delayed infectionof SCBG in mixed culture. Independent of the virus strain, infection of purified SCBG was significantly compared tomixed brain cell cultures. Contrary to this, fibroblasts were infected to the same extent in both culture conditions.There are two main findings of the present study. Firstly, CDV strains displayed differential infection of SCBG inpurified cultures. This is a confirmation and extension of previous findings in mixed culture. Secondly, a dramaticdecrease in the extent of CDV infection was observed after purification of SCBG. This was independent of the CDVstrain used and implies that undetermined interactions between SCBG and other cells, eliminated during purification,crucially determine the susceptibility of SCBG to CDV infection in vitro.

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