The Thai Journal of Veterinary Medicine


Asiatic pennywort [Centella asiatica (L.), Urban, AP] and Indian mulberry (Morinda citrifolia Linn., IM), theAsian plants, have been previously demonstrated in our laboratory that they could markedly enhance the activities ofsome phase II enzymes, but significantly decreased the activities of phase I enzymes, indicating that they may possesscancer chemopreventive potentials. This study was to investigate the anticlastogenic activity of AP and IM against adirect-acting clastogen, mitomycin C (MMC), and indirect-acting clastogens, cyclophosphamide (CYP) and 7, 12-dimethylbenz(a)anthracene (DMBA). Male mice were fed with AP or IM leaves mixing in modified AIN -76 semipurifieddiet, or IM fruit juice or fruit powder solution by gavage, for 2 weeks prior to administration of clastogens. Anticlastogenic effect was determined by using in vivo erythrocytes micronucleus assay. Blood samples werecollected and counted for reticulocytes with and without a micronucleus using the fluorescent microscope. Aftercomparing with the controls, we found that 25% of AP and IM leaves in diets significant decrease micronucleatedperipheral reticulocytes (MNRETs) induced by MMC, CYP and MMC, DMBA, respectively (p<0.05). While IM fruitjuice at 10 and 20 ml/kg BW caused a significant decrease in MNRETs induced only by MMC. IM fruit powder at 100and 500 mg/kg BW decreased MNRETs induced only by MMC in a dose dependent manner, however the significantdecrease was found only in the high dose (p<0.05). These results demonstrated that AP leaves and IM (leaves andfruits) were able to inhibit the clastogenic activity of both direct and indirect-acting clastogens in the mouse,particularly IM leaves which showed the highest inhibitory effect.



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