This experiment was designed to study the results of laboratory diagnosis of Mycoplasma gallisepticum (MG) infection in layer chicken receiving MG vaccines and MG organisms. One hundred and twenty 1-day-old-malelayer chickens were raised in separate isolation rooms and equally allocated into 4 separated groups. When 4 weeks old, groups 1, 2, 3 and 4 served as a negative control, a MG commercially inactivated vaccine group, a MG commercially live vaccine group and a MG infected group, respectively. In each group, ten birds were swabbed and bled when 4, 5, 6, 7, 8, 9 and 11 weeks old. Swab samples were tested for MG Infection by culture and polymerase chain reaction (PCR) procedures. Sera were determined for MG antibody responses by a serum plate agglutination (SPA) test and by 2 commercial enzyme-linked immunosorbant assay (ELISA) test kits, IDEXX®and Synbiotics®. Results revealed that MG organisms were only found in groups 3 and 4 using culture and isolation when 5 and 11 weeks old, and by PCR during the 5 to 9 week old period. The SPA results showed positive reactors in groups 3 and 4 during the 7 to 11 week old period. The positive reactors detected by both commercial ELISA test kits were similar, detecting MG antibody responses in groups 3 and 4 during the 7 to 11 weeks old period. However, only group 2 found positive reactors by the Synbiotic® ELISA test kits, during the 9 to 11 week old period.
Faculty of Veterinary Science, Chulalongkorn University
Pakpinyo, Somsak; Pitayachamrat, Pinyo; Saccavadit, Seubchat; Santaswang, Than; Tawatsin, Achara; and Sasipreeyajan, Jiroj
"Laboratory Diagnosis of Mycoplasma gallisepticum (MG) Infection in Experimental Layer Chicken Receiving MG Vaccines and MG Organisms,"
The Thai Journal of Veterinary Medicine: Vol. 36:
2, Article 2.
Available at: https://digital.car.chula.ac.th/tjvm/vol36/iss2/2