The Thai Journal of Veterinary Medicine
Abstract
Pathogenic Leptospira species cause leptospirosis in animals and humans. Bacterial culture and the serological identification of leptospires are constrined by being time consuming and labor intensive. Oligonucleotide primers complementary to the 16S ribosomal RNA gene sequence of pathogenic leptospires were designed to amplify the 343 bp fragment. The primers were specific to pathogenic Leptospira species and did not amplify either non-pathogenic leptospires or common bacteria found in urine. This developed PCR assay allows the rapid identification of pathogenic leptospires from rodent urine, with high sensitivity and specificity.
DOI
10.56808/2985-1130.2014
First Page
57
Last Page
65
Recommended Citation
Amonsin, Alongkorn; Dumrongwatanapokin, Thanis; Koowatananukul, Chailai; and Wongsawang, Somathat
(2005)
"THE DETECTION OF PATHOGENIC LEPTOSPIRA SPECIES USING A POLYMERASE CHAIN REACTION TEST,"
The Thai Journal of Veterinary Medicine: Vol. 35:
Iss.
3, Article 6.
DOI: https://doi.org/10.56808/2985-1130.2014
Available at:
https://digital.car.chula.ac.th/tjvm/vol35/iss3/6