A DNA fingerprinting technique, repetitive sequence based polymerase chain reaction (rep-PCR), was used to characterize 20 isolates of both pathogenic and nonpathogenic Leptospira spp. Rep-PCR differentiated 20 Leptospira isolates into 16 rep-PCR patterns with a high index of discrimination (D=0.963). The results of cluster analysis demonstrated that pathogenic and nonpathogenic Leptospira belonged to separate clusters with at least 60% similarity. Overall, the results of our studies indicated that rep-PCR is useful for fingerprinting Leptospira species. The technique is simple, rapid, inexpensive and may be used for strain tracking in molecular epidemiological investigations of Leptospirosis outbreaks in the future.
Faculty of Veterinary Science, Chulalongkorn University
Amonsin, Alongkorn; Koowatananukul, Chailai; Damrongwatanapokin, Thanis; and Wongsawang, Somatat
"DNA FINGERPRINTING OF LEPTOSPIRA SPP. USING A REPETITIVE SEQUENCE-BASED POLYMERASE CHAIN REACTION,"
The Thai Journal of Veterinary Medicine: Vol. 33:
1, Article 3.
Available at: https://digital.car.chula.ac.th/tjvm/vol33/iss1/3