THE APPLICATION OF A RT-PCR TECHNIQUE FOR THE DIAGNOSIS OF CANINE DISTEMPER USING PERIPHERAL BLOOD MONONUCLEAR CELLS

Authors

Weerawan Tiwananthakorn
Sirichai Junsuwanaruk
Achariya Sailasuta
Anudep Rungsipipat
Somporn Techangamsuwan
Sapasiri Siripornprasert
Susumu Tateyama
Ryoji Yamaguchi
Kazuyuki Uchida

Abstract

The application of a Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) on 37 canine distemper cases was performed by the amplification of the nucleo-capsid gene derived from the canine distemper virus or CDV-infected mononuclear cells (PBMCs). The animals were divided into 2 groups according to their clinical signs. The first group of animals showed signs of distemper, such as respiratory and/or digestive signs. The second group showed the same signs as first group but with nervous involvements. The findings revealed that 74.1% (20/27) of group 1 tested positive with the RT-PCR assay compared to 60% (6/10) of group 2. These results suggested that RT-PCR is a useful early diagnostic tool for CDV infected animals.

DOI

10.56808/2985-1130.1884

First Page

71

Last Page

81

Recommended Citation

Tiwananthakorn, Weerawan; Junsuwanaruk, Sirichai; Sailasuta, Achariya; Rungsipipat, Anudep; Techangamsuwan, Somporn; Siripornprasert, Sapasiri; Tateyama, Susumu; Yamaguchi, Ryoji; and Uchida, Kazuyuki (2002) "THE APPLICATION OF A RT-PCR TECHNIQUE FOR THE DIAGNOSIS OF CANINE DISTEMPER USING PERIPHERAL BLOOD MONONUCLEAR CELLS," The Thai Journal of Veterinary Medicine: Vol. 32: Iss. 3, Article 5.
DOI: https://doi.org/10.56808/2985-1130.1884
Available at: https://digital.car.chula.ac.th/tjvm/vol32/iss3/5