The objective of the experiment was to study the number of oocytes recovered, their quality and the recovery rate after superovulation, in order to use the oocytes, as a cytoplasm recipient in a nuclear transfer program. A total of 38, New Zealand White mature, doe rabbits were divided into 3 groups, according to the chosen dose of Follicle Stimulating Hormone (FSH); 21 mg (group A), 28 mg (group B) and 40 mg (group C). Five IM injections of FSH were given at 12 hr interval by increasing doses for group A and B and a constant dose for group C. The ovulation was induced by giving 100 iu human chrorionic gonadotropin (hCG) and mating by a vasectomized buck. The oocytes were collected following ovariectomy and direct flushing. The number of corpora lutea (CL), non hemorrhagic and hemorrhagic follicles were counted. The number of CL were 24.5±8.8, 21.3±11.6 and 20.4±8.6 (P>0.05) respectively. The recovery rate in group A, (77.7%) was higher than those of group B, (55.7%) and C, (50.9%) (P<0.001). A higher number of recovered oocytes was found in group A, (19.1±8.0) than those of group B, (11.8±7.0) (P<0.01) and group C, (10.4±8.3) (P<0.001) respectively. More than 90% of recovered oocytes in every group were morphologically normal. This study showed that the dose of FSH influenced the oocyte recovery rate and the number of oocyte per female.
Techakumphu, Mongkol; Numchaisrika, Pranee; Suktrakun, Vaneda; Phanitkitcharoen, Suthai; Kaewnanuer, Astasit; and Suwajanakorn, Somchai
"SUPEROVULATION RESPONSES IN RABBITS TO DIFFERENT DOSES OF FOLLICLE STIMULATING HORMONE,"
The Thai Journal of Veterinary Medicine: Vol. 32:
2, Article 3.
Available at: https://digital.car.chula.ac.th/tjvm/vol32/iss2/3