A PRELIMINARY STUDY ON OOCYTE COLLECTION USING TRANSVAGINAL ULTRASOUND IN SWAMP BUFFALO HEIFERS (BUBALAS BUBALIS)

Authors

Akachart Promdireg
Mongkol Techakumphu
Nawapen Phutikanit
Ancharlie Na-Chiangmai

Abstract

The objective of the study was to investigate the possibility of collecting oocytes using transvaginal ultrasound-guidance, in swamp buffalo heifers, after gonadotropin stimulation. Five swamp buffalo heifers were given a single dose of 3,000 IU of Pregnant Mare Serum Gonadotropin (PMSG) intramuscularly in order to stimulate the ovaries. Examination of the ovarian responses were carried out 48h after stimulation, the follicles were subsequently punctured and the oocytes examine under a stereomicroscope. The results of the ovarian response were 6.6 ± 1.1 follicles per animal with an average diameter of 7.1 ± 4.1 mm. The mean number of oocytes collected per animal was 4.1 ± 2.0 which gave a recovery rate of 63%. The oocytes were classified as cumulus oocyte complexes (COC), 17.2% (5/29); single and partial cumulus oocytes (S+P),10.3% (3/29); denuded cumulus oocytes, 48.3% (14/29); expanded cumulus oocytes (EXP), 3.5% (1/29) and zona pellucida, 20.7%(6/29),respectively. This study shows it is possible to collection the oocytes in swamp buffalo heifers by using a transvaginal, ultrasound-guided, follicle aspiration technique.

DOI

10.56808/2985-1130.1813

First Page

43

Last Page

50

Recommended Citation

Promdireg, Akachart; Techakumphu, Mongkol; Phutikanit, Nawapen; and Na-Chiangmai, Ancharlie (2000) "A PRELIMINARY STUDY ON OOCYTE COLLECTION USING TRANSVAGINAL ULTRASOUND IN SWAMP BUFFALO HEIFERS (BUBALAS BUBALIS)," The Thai Journal of Veterinary Medicine: Vol. 30: Iss. 1, Article 4.
DOI: https://doi.org/10.56808/2985-1130.1813
Available at: https://digital.car.chula.ac.th/tjvm/vol30/iss1/4