Spinal neurones in the thoracolumbar intermediolateral column (IML) of the rat were double-labelled for nitric oxide synthase (NOS) and neurokinin 1 receptor (NK1r) using a double indirect immunofluorescent technique. Immunostaining revealed a colocalisation of NOS and NK1r in a number of the IML neurones, particularly those that had relatively large but scattered cell bodies, and the area closed to lamina X. Neurones that expressed NK1r were more likely to co-express NOS whereas only small number of NOS-expressing neurones were NK1r immunoreactive. From this it may be concluded that the IML neurones, which are principally sympathetic preganglionic neurones, are composed of more than one subgroup. The fact that NK1r and NOS are present in almost entirely different neuronal groups in the dorsal horn whereas significant overlapping occurs in the IML may indicate the discrepancy in neurotransmitter regulation between the somatosensory and the autonomic nervous system.
Suthamnatpong, Ornsiri and Morris, Richard
"CO-EXPRESSION OF NITRIC OXIDE SYNTHASE AND NEUROKININ 1 RECEPTOR IN NEURONES OF THE INTERMEDIOLATERAL COLUMN OF THE RAT SPINAL CORD,"
The Thai Journal of Veterinary Medicine: Vol. 28:
3, Article 6.
Available at: https://digital.car.chula.ac.th/tjvm/vol28/iss3/6