We examined the use of oesphageal-pharyngeal (0/P) samples for the isolation of Foot and Mouth Disease (FMD) virus in animals from which it was difficult to obtain a conventional epithelial tissue specimen. The virus was detected in 42 (28%) of 150 samples, and only 5 (12%) of the 42 cases had positive results when the samples were tested directly with ELISA. The preferred technique for examination of 0/P samples was thus by culturing representative cell samples. The 150 samples came from 22 cases of the disease, with the virus being detected in 16 cases. In 4 of these 16 cases the virus was only detected in 0/P samples. The result indicates that the rate of isolation of the virus will increase if 0/P sampling is regularly used in FMD virus monitoring.
Faculty of Veterinary Science, Chulalongkorn University
Westbury, H.A.; Doughty, W.J.; Chamnanpood, Pornchai; Tangchaitrong, Suchinta; and Forman, A.J.
"Short Communication The Use of Oesophageal - Pharyngeal Samples in the Diagnosis of Food and Mouth Disease,"
The Thai Journal of Veterinary Medicine: Vol. 18:
4, Article 7.
Available at: https://digital.car.chula.ac.th/tjvm/vol18/iss4/7