Sporicidal activity mechanism of lactic acid and peracetic acid on bovine pathogenic Bacillus cereus spores

Authors

• Xin Zhang, Inner Mongolia Minzu University, College of Animal Science and TechnologyFollow
• Yunjiao Chen, Tongliao Agricultural and Animal Husbandry Development CentreFollow
• Jingjing Zhao, Agricultural Radio and Television School of Inner Mongolia Autonomous RegionFollow
• Haiying Zheng, Tongliao Academy of Agricultural and Animal Husbandry SciencesFollow
• Lanlan Liu, Tongliao Agricultural and Animal Husbandry Development CentreFollow
• Wei Liu, Tongliao Agricultural and Animal Husbandry Development CentreFollow
• Mingshan Fu, Tongliao Academy of Agricultural and Animal Husbandry SciencesFollow
• Weijuan Jia, Tongliao Academy of Agricultural and Animal Husbandry SciencesFollow
• Zelin Jia, Inner Mongolia Minzu University, College of Animal Science and TechnologyFollow
• Jiayu Cui, Inner Mongolia Minzu University, College of Animal Science and TechnologyFollow
• Yuhang Zhang, Inner Mongolia Minzu University, College of Animal Science and TechnologyFollow
• Huisheng Xiong, Inner Mongolia Minzu University, College of Animal Science and TechnologyFollow
• Yujie Ma, Inner Mongolia Minzu University, College of Animal Science and TechnologyFollow
• Xueli Wang, Inner Mongolia Minzu University, College of Animal Science and TechnologyFollow
• Haifeng Wang, Tongliao Academy of Agricultural and Animal Husbandry SciencesFollow

Abstract

In recent years, the pathogenicity of Bacillus cereus (B. cereus) has increased significantly, its host adaptability has continued to expand, and the infection spectrum has also expanded. At the same time, food contamination by B. cereus is also common. Therefore, it is of practical significance to select green, safe and effective inhibitors. Lactic acid (LA) and peroxyacetic acid (PAA) possess good antibacterial properties, making them environmentally friendly, safe, and non-toxic. The purpose of this experiment was to preliminarily explore the inhibition mechanism of LA and PAA on B. cereus spores. The effects of LA and PAA on B. cereus spores were evaluated by measuring the MIC and MBC of LA and PAA inhibiting B. cereus spores, the effect of spore germination, the turbidity of spore suspension (OD₆₀₀), the release of macromolecules in spores, the content of ROS in spores, the release of DPA in spores and the hydrophobicity of spore surface. The results showed that the MIC and MBC of LA against spores were 0.315% and 40.375%, respectively, and the MIC and MBC of PAA against spores were 0.030% and 0.119%, respectively. Different concentrations of LA and PAA could decrease the spore germination rate, spore suspension turbidity, and spore hydrophobicity of B. cereus, and also increase the release of macromolecules in B. cereus spores, the content of spore reactive oxygen species (ROS), and the release of spore dipicolinic acid (2,6-pyridinedicarboxylic acid, DPA). The above results showed that LA and PAA could inactivate B. cereus spores by damaging the integrity of the cell membrane and cell wall of spores.

DOI

10.56808/2985-1130.3990

First Page

1

Last Page

10

Recommended Citation

Zhang, Xin; Chen, Yunjiao; Zhao, Jingjing; Zheng, Haiying; Liu, Lanlan; Liu, Wei; Fu, Mingshan; Jia, Weijuan; Jia, Zelin; Cui, Jiayu; Zhang, Yuhang; Xiong, Huisheng; Ma, Yujie; Wang, Xueli; and Wang, Haifeng (2026) "Sporicidal activity mechanism of lactic acid and peracetic acid on bovine pathogenic Bacillus cereus spores," The Thai Journal of Veterinary Medicine: Vol. 56: Iss. 4, Article 1.
DOI: https://doi.org/10.56808/2985-1130.3990
Available at: https://digital.car.chula.ac.th/tjvm/vol56/iss4/1