Fluctuations in lactobacillus and coliforms population in faeces of piglets and antibiotic susceptibility of Escherichia coli strains isolated from stool samples of piglets

Authors

Lan Tran Thi Quynh, Nong Lam UniversityFollow
Giang Hoang Thanh, SagrifoodFollow

Abstract

The aim of study was to assess the intestinal health status of the weaning piglets by analyzing the lactobacillus: coliforms ratio (L/C ratio), the number of E. coli. as the pathogenic indicator, and to determine the antibiotic susceptibility of E. coli isolates. A total of 180 weaned piglets (weaning at 25d of age) of two trials were allotted to 3 treatments (n = 90, 30 piglets/treatment): control (CON, = Basal diet (BD); treatment 1 (T1 = BD + Super-Biotic TP) and treatment 2 (T2= BD + Bergazyme P®). The total lactobacillus, coliforms, and E. coli were counted from fecal samples one day before weaning and four times at the post-weaning stage until 55 days old (31, 37, 44, and 54 d of age). Minimum Inhibitory Concentrations (MICs) were performed by micro-broth dilution and interpreted conforming to standard references. The dynamics of the lactobacilli population as influenced by growth stage and diet supplementation were not significant difference between the 3 treatments. Lactobacillus count in feces of weaned pigs was up to 106 to below 109 cfu/g. The average fecal coliform concentration was at above 105 cfu/g to below 108 cfu/g. The variation of E. coli population was below 107cfu/g at different stages after weaning. No significant differences between treatments were observed in the proportion of piglets with L/C ratio scoring less than 1.3. The most E. coli isolates were MDR (multi- drug resistance) to 12 antimicrobials. The highest prevalence of resistance was to oxytetracycline and amoxicillin with MIC >256 μg/ml accounting respectively for 95.2% and 92.8%, however, 81.9% of isolates were susceptible to cefpodoxime, and 84.3% of isolates were susceptible to ceftiofur. Oxytetracycline belonged to the 32/35 identified resistance patterns. The highest resistance patterns were OXY- STR- AZI- POD- XNL- PEN- FFN- ENRO- DOX- NEO- AMOX- COL with 15.7 % of pan drug resistance (PDR).

DOI

10.56808/2985-1130.3694

First Page

197

Last Page

209

Recommended Citation

Tran Thi Quynh, Lan and Hoang Thanh, Giang (2024) "Fluctuations in lactobacillus and coliforms population in faeces of piglets and antibiotic susceptibility of Escherichia coli strains isolated from stool samples of piglets," The Thai Journal of Veterinary Medicine: Vol. 54: Iss. 2, Article 8.
DOI: https://doi.org/10.56808/2985-1130.3694
Available at: https://digital.car.chula.ac.th/tjvm/vol54/iss2/8