Effect of modified smart extender on sperm longevity and frozen-thawed semen quality in Bantam chicken (Gallus gallus)

Authors

Nattaporn Chaysakul, Faculty of Veterinary Medicine, Chiang Mai UniversityFollow
Naruephon Leeamnuaycharoen, Faculty of Veterinary Medicine, Chiang Mai UniversityFollow
Marvin Bryan Salinas, College of Veterinary Science and Medicine, Central Luzon State University,Follow
Teepakorn Lertwichaikul, Faculty of Veterinary Medicine, Chiang Mai UniversityFollow
Chakorn Kunkaew, Faculty of Agricultural Science and Technology, Rajamangala University of Technology Lanna Lam PangFollow
Jureerat Sumretprasong, Akkhraratchakumari Veterinary College, Walailak UniversityFollow
Anucha Sathanawongs, Faculty of Veterinary Medicine, Chiang Mai University, ThailandFollow

Abstract

The round body shape and short legs of Bantam chickens (Gallus gallus) pose breeding challenges, making artificial insemination critical for these animals. To contribute to such assisted reproductive technology in avians, this study assessed the effect of a modified simple medium for assisted reproductive technology (SMART) extender on sperm longevity and frozen-thawed semen quality compared to the Blumberger Hahnen Sperma Verdünner (BHSV) extender. Sperm motility and viability were measured in 20 roosters at fresh and chilled conditions at 4-, 24-, and 48-hour intervals. For the subsequent freezing step, 6% and 9% DMA were added to both extenders as cryoprotectants. Results showed significant declines in sperm motility and viability over time (P<0.05) using the modified SMART extender, indicating that the extended semen should be used within 4 hours of collection. CASA analysis also found no significant differences in total motility between the different combinations of extenders and cryoprotectant levels (P>0.05). However, notable differences in progressive motility were observed between the modified SMART extender with 6% DMA and the BHSV extender with 6% and 9% DMA (P<0.05). In conclusion, the modified SMART extender effectively extends semen longevity but falls short of the BHSV extender in terms of cryopreservation efficiency.

DOI

10.56808/2985-1130.1086

First Page

183

Last Page

187

Recommended Citation

Chaysakul, Nattaporn; Leeamnuaycharoen, Naruephon; Salinas, Marvin Bryan; Lertwichaikul, Teepakorn; Kunkaew, Chakorn; Sumretprasong, Jureerat; and Sathanawongs, Anucha (2024) "Effect of modified smart extender on sperm longevity and frozen-thawed semen quality in Bantam chicken (Gallus gallus)," The Thai Journal of Veterinary Medicine: Vol. 54: Iss. 2, Article 6.
DOI: https://doi.org/10.56808/2985-1130.1086
Available at: https://digital.car.chula.ac.th/tjvm/vol54/iss2/6