The Thai Journal of Veterinary Medicine


The paper illustrates some aspects and problems of steroids RIA findings, obtained during the studies on the problem of weak estrous in swamp buffaloes. The work described herein which stem from three aspects of steroids RIA; 1) chromatographic technique of steroids separation 2) immunoreactivity of labelled I steroids and displacement efficiency; 3) technique of the separation of free and bound fractions. The nonaffinity and low displacement efficiency are also discussed.

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