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The Thai Journal of Veterinary Medicine

Abstract

Haplorchis taichuiis an intestinal heterophyidfluke that is pathogenic to humans. This parasite can mature in the small intestine of birds and mammals. The purpose of this study was to investigatethe effect of chia seed oil (Salvia hispanica L.) on H. taichuiin vitroand in vivo. The activity of adult H. taichuiworms incubated in Tyrode’s solution containing praziquantel (10μg/ml) or chia oil (1 or 10 mg/ml) for 1, 6, 12 and 24 hours was assessed by observation under a stereo-microscope. Syrian hamsters infected with 200 H. taichuimetacercariae were treated with 25mg/kgBW praziquantel in a single dose or 100mg/kgBW chia oil for 3 days. The number of worms recovered from the small intestine was determined and morphology was examined by scanning electron microscopy (SEM). All the worms were inactive and contracted after 1h exposure to10 μg/ml praziquantel. Twenty percent of the worms were inactive after exposure to 1 mg/ml of chia oil for 12 h and 100% of the worms were inactive after exposure to 10 mg/ml chia oil for 1 h.The average number of adult H. taichuiworms recovered from the intestines of hamsters treated with chia oil was 60 ± 11.57 and SEM analysis showed time and concentration dependent blebbing, swelling and sloughing of the tegument surface. There were no worms recovered from the hamsters treated with praziquantel. The blebbing and sloughing results of chia oil on the H. taichuicovering looked similar to those of praziquantel

Publisher

Faculty of Veterinary Science, Chulalongkorn University

First Page

393

Last Page

400

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