Gene expression profiling of NGF, BDNF, NT-3 and TrkB receptor in the development of prehierarchical follicles of Zi Geese (Anser cygnoides)

Authors

Cornelius Tlotliso Sello
Chang Liu
Yujian Sui
Hui Yang
Ziqiu Wang
Hongtao Lu
Jingtao Hu
Yongfeng Sun

Abstract

The expression patterns of neurotrophins(NTs) at the gene and protein level including nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3),and TrkB receptor were investigated in the prehierarchical follicles of Zi Geese by RT-qPCR and Western blotting analysis. The mRNA levels of NGF, BDNF, NT-3and TrkBreceptor increased significantly in the small white follicles (SWF), and more interesting by BDNFand NT-3transcripts were not detectable in small yellow follicles (SYF) marked as the last stage of prehierarchical ovarian development before transition into preovulatory follicles. Western blot revealed the presence of all the target neurotrophins and high-affinity receptor at the protein level with varying predominance at each stage of follicle classifications. These results indicate that NGF, BDNF, NT-3,and TrkBare implicated in the folliculogenesis and may have an influential effect during the development and growth of the Zi Geese reproductive system.

DOI

10.56808/2985-1130.2987

First Page

235

Last Page

241

Recommended Citation

Sello, Cornelius Tlotliso; Liu, Chang; Sui, Yujian; Yang, Hui; Wang, Ziqiu; Lu, Hongtao; Hu, Jingtao; and Sun, Yongfeng (2019) "Gene expression profiling of NGF, BDNF, NT-3 and TrkB receptor in the development of prehierarchical follicles of Zi Geese (Anser cygnoides)," The Thai Journal of Veterinary Medicine: Vol. 49: Iss. 3, Article 5.
DOI: https://doi.org/10.56808/2985-1130.2987
Available at: https://digital.car.chula.ac.th/tjvm/vol49/iss3/5