Validation of commercially available EIA kit for measurement of feline plasma kisspeptin

Authors

Prattana Tanyapanyachon
Junpen Suwimonteerabutr
Olga Amelkina
Kaywalee Chatdarong

Abstract

Circulating kisspeptin is described to be related with the reproductive system. Astudy of plasma kisspeptin levels, thus, willprovide the better understanding of reproductive endocrinology in the domestic cat. The present study aimed (i) to validate the only available commercial kisspeptin EIA kit which is currently used in humansto be used in domestic catsand (ii) to determine and compare the plasma kisspeptin levels at different reproductive stages. The intra-assay coefficient of variation (CV) was 11.13%. The inter-assay CV ranged from 5.00 –8.25%. The parallel graphs represented the homology between human kisspeptin and feline endogenous kisspeptin. The recovery rate ranged from 78% -106%. To compare the plasma kisspeptin levels among reproductive status, plasma samples were collected from the prepubertal female cats (n = 6) and sexually mature female cats with an inactive(n = 6) and follicular staged (n = 6). Although no statistically significant difference in the plasma kisspeptin concentration between reproductive stages was observed, there was a tendency that the plasma kisspeptin levels were higher in the prepuberal cats compared with pubertal cats. In conclusion, the commercial EIA kit can be used to determine plasma kisspeptin in the domestic cats.

DOI

10.56808/2985-1130.2984

First Page

209

Last Page

215

Recommended Citation

Tanyapanyachon, Prattana; Suwimonteerabutr, Junpen; Amelkina, Olga; and Chatdarong, Kaywalee (2019) "Validation of commercially available EIA kit for measurement of feline plasma kisspeptin," The Thai Journal of Veterinary Medicine: Vol. 49: Iss. 3, Article 2.
DOI: https://doi.org/10.56808/2985-1130.2984
Available at: https://digital.car.chula.ac.th/tjvm/vol49/iss3/2