The aim of the present study was to construct a recombinant strain by using the probiotics Lactobacillus acidophilus expressing the epidermal growth factor (EGF), which can improve intestinal morphology, and to test its effect on the intestinal histology in LPS (lipopolysaccharide)-challenged mice. The EGF gene was cloned into the shuttle vector pSET4s and then transformed into the L. acidophilus MG6243 strain by electroporation. Two recombinant strains were confirmed by SDS-PAGE and named ΔMG6243-1 and ΔMG6243-2. Sixty mice were randomly assigned to six treatments: 1) non-challenged control, 2) LPS-challenged control, 3) LPS-challenged + 200 μl MG6243, 4) LPSchallenged + 200 μl ΔMG6243-1, 5) LPS-challenged + 200 μl ΔMG6243-1 + inducer IPTG, and 6) LPS-challenged + 200 μl ΔMG6243-2. There were ten replicates for each treatment, and each replicate had one mouse. On d 15, the LPSchallenged mice were injected intraperitoneally with LPS at 10 mg/kg BW, while the non-challenged mice were injected with 0.9% NaCl solution. Three hours after the challenge, all mice were killed. Results showed that both ΔMG6243-1 and ΔMG6243-2 could increase villus height in the duodenum, ileum and jejunum (P<0.05). In addition, the recombinant L. acidophilus decreased the crypt depth in the duodenum and ileum (P<0.05). These results indicated that the recombinant L. acidophilus could effectively improve the intestinal histology in LPS-challenged mice.
Faculty of Veterinary Science, Chulalongkorn University
Lv, Yang; Kang, Ping; Zhang, Lin; Li, Xueni; Wang, Lei; Zhao, Di; Yi, Dan; Ding, Binying; Chen, Hongbo; Hou, Yongqing; and Wu, Tao
"Generation of epidermal growth factor and green fluorescent protein coexpressing Lactobacillus acidophilus and its effect on intestinal histology improvement in mature LPS-challenged mice,"
The Thai Journal of Veterinary Medicine: Vol. 48:
2, Article 2.
Available at: https://digital.car.chula.ac.th/tjvm/vol48/iss2/2