Most recently, adipose tissue (AD) has become an alternative source for mesenchymal stem cells (MSCs) instead of the invasive method of bone marrow (BM) aspiration both in human and canine. In this study, we compared MSCs derived from adipose tissue (AD-MSCs) and bone marrow (BM-MSCs) regarding morphology and cell yield for instant usage by using the standard protocol of counting colony forming unit-fibroblast (CFU-F). MSCs from both sources showed fibroblast-like morphology and formed colonies termed as CFU-F after culturing in plastic surface for 10 days. The colony number per mononuclear cells (MNCs) and the colony number per adherent cells derived from AD were significantly higher than those derived from BM. Our study suggested that AD not only was a suitable source to harvest and but also had a higher performance of clonal efficiency of MSCs than BM. Thus, subcutaneous AD might be an appropriated source for stem cells therapy in canine.
Faculty of Veterinary Science, Chulalongkorn University
Tanamai, Nada; Chantakru, Sirirak; and Vijarnsorn, Monchanok
"Cloning Efficiency of Canine Mesenchymal Stem cells Isolated from Bone Marrow of Femoral Head and Subcutaneous Adipose Tissue,"
The Thai Journal of Veterinary Medicine: Vol. 43:
1, Article 4.
Available at: https://digital.car.chula.ac.th/tjvm/vol43/iss1/4