This study was done to evaluate the efficiency of ultrasound-guided transvaginal follicular aspiration without hormonal pre-stimulation for collecting the oocytes of indigenous Thai beef cattle (Bos indicus), and to assess the vitrification method for their preservation. All animals between 2.5-12 years were categorized according to age (i.e. heifer, young cow, and old cow). A medium-sized follicle (φ 3-8 mm.) had the greatest distribution among the various size classes (p≤ 0.05), and the mean of oocytes collected from each group was not significantly different. Postthawed survival was 60.78, 53.70 and 57.50%, with a cleavage rate of 38.71, 37.93 and 39.13%, and a blastocyst embryo of 6.45, 3.45 and 4.35% among heifers, young cows and old cows, respectively, which was significantly less than the fresh control group and solution control group (p≤ 0.05). The present study suggests that OPU in Thai indigenous beef cattle without hormonal pre-stimulation can be routinely done for an extended period of time. The age of donor does not affect the number and quality of oocytes aspirated, post-thawed viability and the number of embryos developing after IVF.
Faculty of Veterinary Science, Chulalongkorn University
Sakhong, Denpong; Vongpralub, Thevin; Katawatin, Suporn; and Sirisathien, Saksiri
"Ultrasound-guided Transvaginal Follicular Aspiration and Development of Vitrified-thawed Thai Indigenous Beef Cattle (Bos indicus) Oocytes after in vitro Fertilization,"
The Thai Journal of Veterinary Medicine: Vol. 42:
4, Article 14.
Available at: https://digital.car.chula.ac.th/tjvm/vol42/iss4/14