The Thai Journal of Veterinary Medicine
Article Title
Abstract
The present study aims to investigate a suitable L-cysteine level to be added the lactose-egg yolk (LEY) extender to improve the quality of boar semen after freezing-thawing using Annexin-V and propidium iodide (PI) assay detected with flow cytometer. Ejaculates from twelve boars were processed in the LEY extender and added with 0, 5, 10 and 15 mM of L-cysteine. Semen suspensions were cryopreserved with a controlled-rate freezer and thawed for examination. The surface of sperm plasma membrane was explored by scanning electron microscopy (SEM) while sperm membrane integrity was performed by Annexin-V/PI assay. Of all treatments, the damaged spermatozoa in various patterns were observed whereas the intact spermatozoa were still found by SEM. The viable and non-apoptotic (AN-/PI-) sperms were higher (p< 0.05) in the levels of 5 and 10 mM. These results indicated that the damaged spermatozoa were found after freezing/thawing and the L-cysteine at concentrations of 5 to 10 mM added to LEY extender was the optimal dose against oxidative stress and acquisition for improving cryopreserved boar sperm quality.
Publisher
Faculty of Veterinary Science, Chulalongkorn University
First Page
483
Last Page
488
Recommended Citation
Tienthai, Paisan; Chanapiwat, Panida; Manee-in, Sukanya; Gronsang, Dulyatad; and Kaeoket, Kampon
(2012)
"Assessment of Appropriate L-cysteine Concentration for Boar Semen Cryopreservation by Using Flow Cytometry,"
The Thai Journal of Veterinary Medicine: Vol. 42:
Iss.
4, Article 11.
Available at:
https://digital.car.chula.ac.th/tjvm/vol42/iss4/11