Assessment of Appropriate L-cysteine Concentration for Boar Semen Cryopreservation by Using Flow Cytometry

Authors

Paisan Tienthai
Panida Chanapiwat
Sukanya Manee-in
Dulyatad Gronsang
Kampon Kaeoket

Abstract

The present study aims to investigate a suitable L-cysteine level to be added the lactose-egg yolk (LEY) extender to improve the quality of boar semen after freezing-thawing using Annexin-V and propidium iodide (PI) assay detected with flow cytometer. Ejaculates from twelve boars were processed in the LEY extender and added with 0, 5, 10 and 15 mM of L-cysteine. Semen suspensions were cryopreserved with a controlled-rate freezer and thawed for examination. The surface of sperm plasma membrane was explored by scanning electron microscopy (SEM) while sperm membrane integrity was performed by Annexin-V/PI assay. Of all treatments, the damaged spermatozoa in various patterns were observed whereas the intact spermatozoa were still found by SEM. The viable and non-apoptotic (AN-/PI-) sperms were higher (p< 0.05) in the levels of 5 and 10 mM. These results indicated that the damaged spermatozoa were found after freezing/thawing and the L-cysteine at concentrations of 5 to 10 mM added to LEY extender was the optimal dose against oxidative stress and acquisition for improving cryopreserved boar sperm quality.

DOI

10.56808/2985-1130.2428

First Page

483

Last Page

488

Recommended Citation

Tienthai, Paisan; Chanapiwat, Panida; Manee-in, Sukanya; Gronsang, Dulyatad; and Kaeoket, Kampon (2012) "Assessment of Appropriate L-cysteine Concentration for Boar Semen Cryopreservation by Using Flow Cytometry," The Thai Journal of Veterinary Medicine: Vol. 42: Iss. 4, Article 11.
DOI: https://doi.org/10.56808/2985-1130.2428
Available at: https://digital.car.chula.ac.th/tjvm/vol42/iss4/11